Polar Lipid Fraction from Golden Oyster Mushrooms (Pleurotus citrinopileatus) Suppresses Colon Injuries from Inflammatory Stresses in vivo and in vitro
| Autor: | Shinji Yamashita, Mikio Kinoshita, Miho Inobe, Mirinthorn Jutanom, Satoshi Matsumoto, Takuya Seino |
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| Rok vydání: | 2020 |
| Předmět: |
Lipopolysaccharide
030309 nutrition & dietetics General Chemical Engineering Inflammation Pharmacology 03 medical and health sciences chemistry.chemical_compound 0404 agricultural biotechnology Pleurotus citrinopileatus In vivo medicine Colitis 0303 health sciences biology Chemistry 04 agricultural and veterinary sciences General Medicine General Chemistry medicine.disease biology.organism_classification 040401 food science Sphingolipid In vitro Apoptosis lipids (amino acids peptides and proteins) medicine.symptom |
| Zdroj: | Journal of Oleo Science. 69:751-757 |
| ISSN: | 1347-3352 1345-8957 |
| Popis: | The rising incidence of inflammatory bowel disease (IBD) in East Asian countries has necessitated the implementation of preventive methods in the form of dietary supplementation and changes in dietary habits. We have previously reported that dietary golden oyster mushroom (Pleurotus citrinopileatus) ethanol extract (GOMEE) suppresses intestinal inflammation in mouse models of IBD induced by dextran sulfate sodium salt (DSS). Here, we investigated the components of GOMEE that exert suppressive effects on colon inflammation in vivo and in vitro. The total lipid fraction was extracted from GOMEE, and the polar and neutral lipid fractions were subsequently separated via solvent fractionation. Mice were assigned to dietary groups-control, 1% total lipid, 1% polar lipid, or 1% neutral lipid diet-and fed the respective diets for one week; mice were administered 1.5% DSS in drinking water ad libitum for 20 days. Dietary supplementation with the total or polar lipid fraction alleviated DSS-induced chorionic crypt injury as determined by morphological observation, while dietary supplementation with the neutral lipid fraction did not produce such effects. In the in vitro study, using differentiated Caco-2 cells as the colon model, treatment with the total or polar lipid fraction suppressed cell decrease by lipopolysaccharide (LPS)-induced apoptosis whereas treatment with the neutral lipid fraction did not. Moreover, accumulation of glucosylceramide (GlcCer), a fungal sphingolipid, was observed in the intestinal cells after treatment with polar lipid fraction. These results suggest that the active components of GOMEE that suppress colon inflammation are polar lipids, especially GlcCer. The structure of mushroom GlcCer differs from that of the plant counterpart and is therefore expected to exert different food functions. |
| Databáze: | OpenAIRE |
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