Formation of Cholic Acid from 3α,7α,12α-Trihydroxy-5β-Cholestanoic Acid in Human Skin Fibroblasts

Autor:	Mizuho Une, Tsuneo Imanaka, Noboru Izumi, Takahiko Hoshita
Rok vydání:	1992
Předmět:	Bile acid medicine.drug_class Cholic acid Human skin General Medicine Peroxisome Biochemistry chemistry.chemical_compound medicine.anatomical_structure Biosynthesis chemistry medicine Specific activity NAD+ kinase Fibroblast Molecular Biology
Zdroj:	The Journal of Biochemistry. 112:576-577
ISSN:	1756-2651 0021-924X
DOI:	10.1093/oxfordjournals.jbchem.a123942
Popis:	Whether 3 alpha, 7 alpha, 12 alpha-trihydroxy-5 beta-cholestanoic acid (THCA) was converted into cholic acid in human skin fibroblasts was examined. THCA was incubated with subcellular fractions of cultured skin fibroblasts in the presence of NAD+, ATP, CoA, and Mg2+. The reaction products were analyzed by thin-layer chromatography and high-performance liquid chromatography after p-bromophenacyl ester derivatization. The highest specific activity was found in the light mitochondrial fraction (2.71 nmol/mg protein/h). The specific activity was about 9-fold higher than that in heavy mitochondrial fraction. The peroxisomal fraction prepared from the light mitochondrial fraction by sucrose gradient centrifugation was also able to catalyze the conversion of THCA into cholic acid. The specific activity in this fraction was a further 2.2-fold higher than that in the light mitochondrial fraction. These results suggest that cultured human skin fibroblasts are able to convert THCA into cholic acid, and that the activity exists in peroxisomes.
Databáze:	OpenAIRE
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