Effects of temperature on germination of sporangia, infection and protein secretion by Phytophthora kernoviae

Autor: Timothy L. Widmer, B. A. Shelley, Wesley M. Garrett, Douglas G. Luster, Michael B. McMahon
Rok vydání: 2017
Předmět:
Zdroj: Plant Pathology. 67:719-728
ISSN: 0032-0862
DOI: 10.1111/ppa.12782
Popis: Phytophthora kernoviae is reported to be a pathogen on a wide range of plants, but there is little knowledge of the optimal infection conditions. Rhododendron ponticum leaves were inoculated with six different isolates of P. kernoviae sporangia and set at different temperatures from 10-28°C. After 1 week, lesion development and pathogen recovery were only observed from all isolates at 15 and 20°C and a few isolates at 10°C. In an experiment with a more narrow temperature range of 20-25°C, lesion development and pathogen recovery on R. ponticum, Magnolia stellata, and Viburnum tinus occurred consistently at 20 and 21°C, was limited at 22°C, and did not occur at all at 23°C and above. There was no difference in sporangia and zoospore germination at 20-25°C. In a temperature fluctuation experiment, the necrotic area of inoculated R. ponticum leaves was larger the longer leaves were stored at 20°C and smaller the longer leaves were stored at 24°C. To determine if temperature affects secreted proteins that are involved in pathogenicity, crude purifications of secreted proteins from P. kernoviae cultures grown at 20 and 24°C were compared. When spot tested on R. ponticum leaves, the crude protein suspension isolated from cultures grown at 20°C induced necrosis while the proteins secreted from a culture grown at 24°C did not. Proteomic analysis confirmed a 10 kDa protein secreted at both 20 and 24°C shared sequence homology to the conserved domains of known elicitins of other Phytophthora spp. The protein secreted at 20°C that was responsible for the necrosis has not been identified. This article is protected by copyright. All rights reserved.
Databáze: OpenAIRE
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