Scaffold structure and fabrication method affect proinflammatory milieu in three-dimensional-cultured chondrocytes
| Autor: | Carrie K. Hui, Roshni S. Rainbow, David L. Kaplan, Dana M. Cairns, Rucsanda C. Preda, Heenam Kwon, Li Zeng, Lin Sun |
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| Rok vydání: | 2014 |
| Předmět: |
Scaffold
Materials science Cartilage Metals and Alloys Biomedical Engineering Bone matrix Cartilage tissue engineering Cell biology Proinflammatory cytokine Biomaterials medicine.anatomical_structure Water uptake Ceramics and Composites medicine Tumor necrosis factor alpha Biomedical engineering Cartilage tissues |
| Zdroj: | Journal of Biomedical Materials Research Part A. 103:534-544 |
| ISSN: | 1549-3296 |
| Popis: | Cartilage tissue engineering has emerged as an attractive therapeutic option for repairing damaged cartilage tissue in the arthritic joint. High levels of pro-inflammatory cytokines present at arthritic joints can cause cartilage destruction and instability of the engineered cartilage tissue, and thus it is critical to engineer strong and stable cartilage that is resistant to the inflammatory environment. In the present study, we demonstrate that scaffolding materials with different pore sizes and fabrication methods influence the microenvironment of chondrocytes and the response of these cells to pro-inflammatory cytokines, IL-1β and TNFα. Silk scaffolds prepared using the organic solvent hexafluoroisopropanol (HFIP) as compared to an aqueous-based method, as well as those with larger pore sizes, supported the deposition of higher cartilage matrix levels and lower expression of cartilage matrix degradation-related genes, as well as lower expression of endogenous pro-inflammatory cytokines IL-1β in articular chondrocytes. These biochemical properties could be related to the physical properties of the scaffolds such as the water uptake and the tendency to leach or adsorb pro-inflammatory cytokines. Thus, scaffold structure may influence the behavior of chondrocytes by influencing the microenvironment under inflammatory conditions, and should be considered as an important component for bioengineering stable cartilage tissues. |
| Databáze: | OpenAIRE |
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