New dip-and-read test for determining leukocytes in urine

Autor:	J A Cattell, L R Stover, Paul F Corey, J H Pendergrass, R. W. Trimmer, J P Albarella, R J Tyhach, A. E. Burkhardt, A C Skjold, M P Farinelli
Rok vydání:	1987
Předmět:	Pathology medicine.medical_specialty Reagent strip Chromatography Chemistry Biochemistry (medical) Clinical Biochemistry Alcohol Urine Esterase Clinical study Matrix (chemical analysis) Hydrolysis chemistry.chemical_compound Reagent medicine
Zdroj:	Clinical Chemistry. 33:1242-1245
ISSN:	1530-8561 0009-9147
DOI:	10.1093/clinchem/33.7.1242
Popis:	A new reagent strip for the determination of leukocytes in urine (LEUKOSTIX; Ames) is described. The test is based on the esterase activity in leukocytes as a marker. Upon contact between the reagent matrix and a urine containing leukocytes, an amino acid ester is hydrolyzed by the esterase to its corresponding alcohol. The free alcohol then couples with a diazonium salt to produce a purple azo dye. The relative concentration of leukocytes in the urine is obtained by visually comparing the strip reaction with a color chart. Performance of the strip was evaluated in a clinical study involving eight different sites and 867 urine specimens. The comparison method was sediment microscopy; specimens containing five cells or more per high-power field were considered to be positive. Sensitivity was 76.3%, specificity 80.8%. Performance was comparable with that of the CHEMSTRIP LN (Boehringer-Mannheim Diagnostics, Inc.) leukocyte test, which we evaluated concurrently.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::227adb4136c03b1e88e62f168729efc3 https://doi.org/10.1093/clinchem/33.7.1242 Zobrazit plný text záznamu