| Autor: |
M. Anafi, G. Klein, Dov Sulitzeanu, Ada Hatzubai |
| Rok vydání: |
1987 |
| Předmět: |
|
| Zdroj: |
Epstein-Barr Virus and Human Disease ISBN: 9781461289401 |
| DOI: |
10.1007/978-1-4612-4590-2_56 |
| Popis: |
A radioimmunoassay (RIA) has been developed and used to determine the expression of LMP (the membrane protein encoded by the LT3 region of the EBV genome) in cell lines of various origin. The RIA employed microtiter plates coated with LT3FP — a fusion protein consisting of the carboxy half of the LMP molecule and of beta-galactosidase, expressed in E. coli (1). Specifically purified rabbit anti LT3FP antibodies bound to the plates and this binding could be inhibited by LMP positive cell extracts. Linear dose-response curves were obtained over the range 7 – 180 ug protein/ml. The RIA was highly sensitive, specific and reproducible. It could detect 4 ng LMP/mg protein, which corresponded to about 4000 molecules per cell. The specificity of the assay was confirmed by the results of the tests carried out with EBV negative cell extracts — all of them were negative. On the other hand, 18 of 22 EBV carrying cells were LMP positive. Two of 3 EBV negative Burkitt’s lymphoma lines converted by EBV infection in vitro became LMP positive. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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