Coupling of Open Tubular Liquid Chromatography to Electrospray Mass Spectrometry with a Nanospray Interface

Autor:	Gustaf Hulthe, Elisabet Fogelqvist, and Maria A. Petersson
Rok vydání:	1999
Předmět:	Coupling Electrospray Chromatography Chemistry Capillary action Electrospray mass spectrometry Interface (computing) Electrospray ionization Analytical chemistry High voltage Mass spectrometry Analytical Chemistry
Zdroj:	Analytical Chemistry. 71:2915-2921
ISSN:	1520-6882 0003-2700
DOI:	10.1021/ac981352f
Popis:	The concept of interfacing open tubular liquid chromatography (OTLC) to electrospray ionization mass spectroscopy (ESI-MS) is here introduced. This combination has the potential of becoming a powerful and generic tool for separation and quantification of various complex mixtures. We can demonstrate here how easy and straightforward it is to connect an open capillary LC column to nano-ESI, simply by drawing the column through a stainless steel tube onto which the high voltage (HV) is supplied. This method is compared with applying the HV to a gold-coated column tip and the standard dynamic nanospray interface supplied by the ESI-MS manufacturer. Reliability and stability are documented. OTLC-ESI-MS is demonstrated by separation of fatty acids in the negative mode.
Databáze:	OpenAIRE
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