Fluorescence characteristics and photophysical parameters of light-harvesting chlorophyll a/b complex aggregates
Autor: | Evgeny E. Ostroumov, M. S. Khristin, V. Z. Pashchenko, V. V. Fadeev, V. B. Tusov |
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Rok vydání: | 2007 |
Předmět: | |
Zdroj: | Biophysics. 52:462-467 |
ISSN: | 1555-6654 0006-3509 |
Popis: | Fluorescence characteristics and molecular photophysical parameters of light-harvesting chlorophyll a/b complexes isolated from pea were studied in relation to their aggregation state. The aggregate size was varied by changing the Triton X-100 concentration from 0 to 0.23 mM at a chlorophyll concentration of 2.45 μg/ml. Molecular photophysical parameters were determined with laser fluorimetry. Dispersion of large aggregates into smaller ones drastically decreased the intensity of low-temperature (77 K) fluorescence at 700 nm, reduced the singlet-singlet annihilation rate by more than two orders of magnitude, and prolonged the fluorescence lifetime from 0.16 to 3.2 ns. |
Databáze: | OpenAIRE |
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