Fluorescence characteristics and photophysical parameters of light-harvesting chlorophyll a/b complex aggregates

Autor: Evgeny E. Ostroumov, M. S. Khristin, V. Z. Pashchenko, V. V. Fadeev, V. B. Tusov
Rok vydání: 2007
Předmět:
Zdroj: Biophysics. 52:462-467
ISSN: 1555-6654
0006-3509
Popis: Fluorescence characteristics and molecular photophysical parameters of light-harvesting chlorophyll a/b complexes isolated from pea were studied in relation to their aggregation state. The aggregate size was varied by changing the Triton X-100 concentration from 0 to 0.23 mM at a chlorophyll concentration of 2.45 μg/ml. Molecular photophysical parameters were determined with laser fluorimetry. Dispersion of large aggregates into smaller ones drastically decreased the intensity of low-temperature (77 K) fluorescence at 700 nm, reduced the singlet-singlet annihilation rate by more than two orders of magnitude, and prolonged the fluorescence lifetime from 0.16 to 3.2 ns.
Databáze: OpenAIRE