Application of the analytical transmission electron microscopy techniques for detection, identification and visualization of localization of nanoparticles of titanium and cerium oxides in mammalian cells

Autor: Konstantin V. Shaitan, M. V. Erokhina, Alexey V. Feofanov, A. G. Bogdanov, Pavel I. Semenyuk, A. S. Shebanova, Mikhail P. Kirpichnikov, Tatiana Ismagulova, V. I. Muronets, G. E. Onishchenko
Rok vydání: 2014
Předmět:
Zdroj: Biophysics. 59:284-292
ISSN: 1555-6654
0006-3509
DOI: 10.1134/s0006350914020237
Popis: This work represents the results of the study on applicability of the modern methods of analytical transmission electron microscopy (TEM) for detection, identification and visualization of localization of nanoparticles of titanium and cerium oxides in A549, human lung adenocarcinoma cell line. Comparative analysis was performed for images of the nanoparticles in cells obtained in the bright-field mode of TEM, bright-field scanning TEM, and high-angle annular dark field scanning TEM. For identification of nanoparticles in the cells, the analytical techniques, energy-dispersive X-ray spectroscopy and electron energy loss spectroscopy, were compared when used in the modes of obtaining energy spectra from different particles and of element mapping. It was shown that electron tomography is applicable to confirm that nanoparticles are localized in the sample rather than brought in by contamination. The possibilities and fields of using different techniques of analytical TEM for detection, visualization and identification of nanoparticles in biological samples are discussed.
Databáze: OpenAIRE
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