Changes in Body Composition and FTO Whole Blood DNA Methylation Among Japanese Women: A Randomized Clinical Trial of Weight-Loss Program
| Autor: | Yutaka Kimura, Satoshi Kurose, Aya Fujii, Nana Takao, Hiromi Tsutsumi, Sawako Yoshiuchi, Haruhiko Nishida, Katsuko Onishi, Takumi Miyauchi |
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| Rok vydání: | 2020 |
| Předmět: |
Pharmacology
business.industry Physiology 030209 endocrinology & metabolism Methylation 030204 cardiovascular system & hematology medicine.disease Obesity law.invention 03 medical and health sciences 0302 clinical medicine Randomized controlled trial law DNA methylation Internal Medicine medicine Outpatient clinic Epigenetics business Body mass index Whole blood |
| Zdroj: | Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy. 13:2157-2167 |
| ISSN: | 1178-7007 |
| DOI: | 10.2147/dmso.s248769 |
| Popis: | Objective DNA methylation is an epigenetic mechanism that regulates gene expression. The obesity-related (FTO) gene is the first gene found to be associated with fat mass and obesity. However, no studies have examined the relationship between weight-loss intervention effect and FTO methylation in obese individuals with whole blood DNA. The purpose of this study was to quantify FTO whole blood DNA methylation and investigate the relationship between body composition, exercise capacity, and blood parameters with a 6-month weight-loss program intervention. Participants and methods Eighteen female participants (mean age, 50.6 ±12.1 years, body mass index (BMI), 33.5 ± 6.2 kg/m2) who completed a 6-month weight-loss program at the obesity outpatient department at the Health Science Center of Kansai Medical University Hospital from March 2017 to October 2018 were included in the analysis. Participants were randomized into a normal treatment group (NTG) and a group with additional resistance training (RTG). Body composition, exercise tolerance and metabolic index were measured in each participant. DNA methylation status in whole blood samples was determined using pyrosequencing. All measurements were taken during the first visit and at the 6-month post-intervention visit. Results The methylation rate was significantly decreased in the NTG in CpG1 (p=0.011) and total value of CpG (p=0.011), whereas in the treatment group containing resistance training (RTG), CpG3 (p=0.038) was increased significantly. Furthermore, the independent factors that determine %CpG3 of RTG were visceral fat area change rate (%VFA) (β = -0.568, P = 0.007, R2 = 0.527) and resistance training (β = 0.517, P = 0.012, R2 = 0.527), which have been extracted. Conclusion A 6-month weight-loss program, including resistance training, may be associated with decreased visceral fat area changes and increased RTG CpG3 methylation changes. However, further replication studies with larger sample sizes are warranted to verify the findings of this study. |
| Databáze: | OpenAIRE |
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