Effect of m-carbonyl cyanide 3-chlorophenylhydrazone on inorganic polyphosphates synthesis in Saccharomyces cerevisiae under different growth conditions

Autor: O. Yu. Kochetkova, A. P. Il'chenko, Igor S. Kulaev, L. V. Trilisenko, V. M. Vagabov
Rok vydání: 2011
Předmět:
Zdroj: Microbiology. 80:15-20
ISSN: 1608-3237
0026-2617
DOI: 10.1134/s0026261711010176
Popis: The effects of different concentrations of the protonophore uncoupler m-carbonyl cyanide 3-hchlorophenylhydrazone (CCCP) on the synthesis of inorganic polyphosphates (polyP) during the first 0.5 h of hypercompensation in the yeast Saccharomyces cerevisiae VKM Y-1173 growing on media with 2% glucose under low (hypoxia) or high aeration or with 1% (vol/vol) ethanol under high aeration were studied. It was shown that the yeast growth on ethanol was completely inhibited by 5 μM CCCP, while growth on glucose was inhibited by 25 μM CCCP, independently of aeration of the medium. The maximum rate of H2 absorption was shown at 2, 5, and 25 μM CCCP for the cells grown on ethanol, on glucose under high aeration, and on glucose under hypoxia, respectively. Against the decrease of total ATP level and total polyP, CCCP had a nonuniform effect on the synthesis of individual polyP fractions. CCCP maximally inhibited synthesis of the most actively formed fractions: polyPI during growth on glucose under hypoxia, polyPIII during growth on glucose under aeration, and polyPIII and polyPV during growth on ethanol. CCCP had no substantial effect on the synthesis of polyPII and polyPIV fractions, the formation of which seems to be less related to the electrochemical potential gradient of H+ ions.
Databáze: OpenAIRE
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