Reevaluation of the 'in vitro' and 'in vivo' methods of nitrate reductase determination in pea roots

Autor:	J. Sahulka
Rok vydání:	1985
Předmět:	chemistry.chemical_classification Cadmium food and beverages chemistry.chemical_element Plant Science Horticulture Nitrate reductase In vitro chemistry.chemical_compound Enzyme Nitrate chemistry Biochemistry In vivo Nitrite
Zdroj:	Biologia Plantarum. 27:424-431
ISSN:	1573-8264 0006-3134
DOI:	10.1007/bf02894711
Popis:	Nitrate consumption, nitrite production and nitrite loss were followed in the“in vitro” nitrate reductase (NR) assay mixtures with pea root extracts, and nitrite production in the “in vitro” NR assay was compared with anaerobic nitrite production in the “in vivo” assay with pea root segments. NR activity could not be reliably determined on the basis of nitrate consumption using three methods of NO3-determination (salicylate, cadmium, enzymatic). NR activity based on nitrite determination was slightly underestimated due to nitrite loss (less than 10% with pea root extracts) in the assay mixture. Nitrite production in the “in vivo” NR assay under optimum conditions closely resembled nitrite production in the “in vitro” NR assay and thus reflected NR level in the tissue.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::2237213990d680e1b0d26f82c440c700 https://doi.org/10.1007/bf02894711 Zobrazit plný text záznamu Full text from SpringerLink