Effect of Lactobacillus rhamnosus GR-I Supernatant on Cytokine and Chemokine Output From Human Amnion Cells Treated With Lipoteichoic Acid and Lipopolysaccharide
| Autor: | Gregor Reid, John R. G. Challis, Alan D. Bocking, Rebecca J E Koscik, Wei Li, Sung Ouk Kim |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Chemokine Lipopolysaccharide medicine.medical_treatment Microbiology Andrology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Lactobacillus rhamnosus medicine Interleukin 6 030219 obstetrics & reproductive medicine biology Amnion Obstetrics and Gynecology biology.organism_classification 3. Good health 030104 developmental biology Cytokine medicine.anatomical_structure chemistry biology.protein lipids (amino acids peptides and proteins) Tumor necrosis factor alpha Lipoteichoic acid |
| Zdroj: | Reproductive Sciences. 25:239-245 |
| ISSN: | 1933-7205 1933-7191 |
| Popis: | Preterm birth occurs in 9% to 13% of all human pregnancies and accounts for 80% of all neonatal morbidities and mortalities. Approximately 40% of all preterm births are idiopathic and about half are associated with infection and/or an activated inflammatory process. Further to studies showing anti-inflammatory effects of supernatant from the probiotic Lactobacillus rhamnosus GR-1 (GR-1), we tested its ability to modulate cytokine and chemokine production from amnion cells in response to stimulation by bacterial wall components, lipopolysaccharide (LPS), and lipoteichoic acid (LTA). Placentae were collected from women undergoing elective cesarean section at term. Amnion cells were cultured for 48 hours to confluence, serum starved for 12 hours, and then treated with GR-1 supernatant (1:20 dilution), followed after 12 hours by LPS (100 ng/mL) or LTA (10 ng/mL) for an additional 12 hours. Both LTA and LPS caused significant increases in the concentration of the pro-inflammatory cytokine, tumor necrosis factor α (TNF-α; 103.9 ± 67.5 pg/mL and 368.3 ± 65.7 pg/mL, respectively) in medium from cultured amnion cells compared to control ( |
| Databáze: | OpenAIRE |
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