THU0328 A Comprehensive Evaluation of Serum Autoantibodies in Primary Sjögren's Syndrome
| Autor: | Sandrine Jousse-Joulin, Yves Renaudineau, G. Carvajal Alegria, B. Bendaoud, A. Saraux, J.-O. Pers, C. Capaldo, Valérie Devauchelle-Pensec |
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| Rok vydání: | 2016 |
| Předmět: |
musculoskeletal diseases
Tissue transglutaminase Immunology Population General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound stomatognathic system Rheumatology Cardiolipin Immunology and Allergy Medicine education education.field_of_study biology medicine.diagnostic_test business.industry Autoantibody Hypergammaglobulinemia medicine.disease stomatognathic diseases chemistry Erythrocyte sedimentation rate biology.protein Antibody business Gliadin |
| Zdroj: | Annals of the Rheumatic Diseases. 75:306.1-306 |
| ISSN: | 1468-2060 0003-4967 |
| Popis: | Background Conventional antibodies are well described and studied in primary Sjogren syndrome, nevertheless patients can also present unconventional autoantibodies. Objectives The objective of this study was to investigate the prevalence of a large panel of conventional and unconventional autoantibodies (Ab) in a well-characterized population of patients with primary Sjogren9s syndrome (pSS). Methods Two hundred and twenty six pSS patients benefiting from a comprehensive clinical and radiological assessment were recruited. A new technique based on chemiluminescence (ISYS-IDS) was selected, in 44 patients, to assay 17 autoantigens including SSA/Ro (52+60 kDa), SSB/La, dsDNA, centromere, Sm, U1-snRNP, Scl70, Jo1, cardiolipin, β2-GPI, CCP2, PR3, MPO, GBM, transglutaminase (IgA), and deaminated gliadin (IgA&IgG). If positive, the presence of Ab was validated by another technique. The study was completed by the search for anti-nuclear Ab (ANA) on HEp-2 cells and rheumatoid factors (RF) against human and rabbit IgG. Results Search for conventional Ab showed ANA (71.7% at 1:320), anti-SSA/Ro Ab (61.1%), anti-SSB/La Ab (31.0%, all anti-SSA+) and IgM RF (44.2%). The triple Ab association (ANA+SSA+RF) rather than the SSA/SSB association was associated with an earlier diagnosis, an active immunological profile (hypergammaglobulinemia, erythrocyte sedimentation rate) and a salivary gland involvement (lymphocyte infiltration, ultrasonography and enlargement). Three patients out of 44 (6.8%) had unintended Ab in current practice directed against centromere, Jo1, and dsDNA plus IgG deaminated gliadin Ab but without associated clinical signs. Conclusions The ability to combine a wide range of autoantibodies can lead to defining new subgroups of pSS patients for precise disease characterization, patient stratification and response prediction. Disclosure of Interest None declared |
| Databáze: | OpenAIRE |
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