A novel method for direct detection of Bacillus cereus toxin genes in powdered dairy products
| Autor: | Margarita M. Correa, Angel E. Aceves-Diez, Jennifer A. Sánchez-Chica, Laura M. Castañeda-Sandoval |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Food poisoning
biology Chemistry Toxin 0402 animal and dairy science Bacillus cereus 04 agricultural and veterinary sciences Enterotoxin biology.organism_classification medicine.disease_cause medicine.disease 040401 food science 040201 dairy & animal science Applied Microbiology and Biotechnology DNA extraction Microbiology 0404 agricultural biotechnology Infant formula Multiplex polymerase chain reaction medicine Gene Food Science |
| Zdroj: | International Dairy Journal. 103:104625 |
| ISSN: | 0958-6946 |
| DOI: | 10.1016/j.idairyj.2019.104625 |
| Popis: | Bacillus cereus is a major foodborne pathogen with the potential of causing emetic and diarrhoeal food poisoning. This study reports on the development of a sensitive and reliable method that involves direct DNA extraction from powdered milk and infant formula and a multiplex PCR for detection of four B. cereus toxin genes (nheA, hblD, cytK2 and cesB). Detection limits were 2 × 102 cfu g−1 for enterotoxin genes and 2 × 103 cfu g−1 for both the enterotoxin and emetic genes. Determination of B. cereus toxin genes in powdered dairy food samples revealed five toxigenic groups. Groups I to IV contained enterotoxin genes while group V, enterotoxin and emetic genes. The results indicate that the molecular method developed is a rapid and efficient diagnostic tool for direct detection and monitoring of B. cereus toxin genes in powdered milk and infant formula and possibly in other powdered foods. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect