EXPLORING THE CHRONIC MORTALITY AFFECTING ABALONES IN TAIWAN: DIFFERENTIATION OF ABALONE HERPESVIRUS-ASSOCIATED ACUTE INFECTION FROM CHRONIC MORTALITY BY PCR AND IN SITU HYBRIDIZATION AND HISTOPATHOLOGY
| Autor: | Pen-Heng Chang, Shu-Ting Kuo, Min-Shou Chen, Tristan Renault, I-Wen Chen, Chiung-Hsiang Cheng, Meei-Mei Chen |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Gonad Abalone 040301 veterinary sciences 04 agricultural and veterinary sciences In situ hybridization Biology biology.organism_classification Virology Virus 0403 veterinary science Pathogenesis 03 medical and health sciences 030104 developmental biology medicine.anatomical_structure medicine Histopathology Tropism Haliotis diversicolor |
| Zdroj: | Taiwan Veterinary Journal. 42:1-9 |
| ISSN: | 2382-5588 1682-6485 |
| DOI: | 10.1142/s1682648515500237 |
| Popis: | Abalone herpesvirus (AbHV) infection of cultured abalones Haliotis diversicolor supertexta induced acute high mortality in 2003. Years later, sporadic mortality was noted for an extended period of months, resulting in high cumulative mortality. Moribund abalones were analyzed using PCR, in situ hybridization, and histopathology, because thus far no viral particles have been observed by transmission electron microscopy. PCR using 20 primer sets, specifically designed from sequences of acute AbHV infection, failed to amplify any products from abalones suffering from chronic mortality. Subsequently, a 1406-bp sequence was amplified from chronic moribund abalones, and this sequence showed a 92% (553 bp/602 bp) homology with the gene of an AbHV Taiwan isolate (NCBI serial no. KF537536.1), suggestive of an AbHV pathotype. Histopathology of AbHV pathotype infection showed hemocyte infiltration in the lamina propia of the digestive tract, and hemocytes of various stages were evident, as well as the loss of seminal tubules in the gonad. In situ hybridization revealed that in AbHV infection, positive signals were restricted to the neural ganglia, while in AbHV pathotype infection, positive signals were observed only in the hemocytes. It appeared that the tropism of AbHV shifted from mainly neurotropic in AbHV infection to mainly hemocytotropic in abalone suffering from chronic mortality. Abalone shriveling syndrome-associated virus co-infection was detected in some of AbHV pathotype infection events. Further studies are needed to better understand the pathogenesis of AbHV pathotype affecting H. diversicolor in Taiwan. |
| Databáze: | OpenAIRE |
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