Design principles for lymphatic drainage of fluid and solutes from collagen scaffolds
| Autor: | Gavrielle M. Price, Emily A. Margolis, Joe Tien, Brent J. Coisman, Rebecca L. Thompson, Keith H. K. Wong, Tyler Ryan |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Materials science government.form_of_government Metals and Alloys Biomedical Engineering Design elements and principles 02 engineering and technology 021001 nanoscience & nanotechnology Biomaterials Endothelial stem cell 03 medical and health sciences Lymphatic Endothelium 030104 developmental biology Lymphatic system Ceramics and Composites government Drainage Current (fluid) 0210 nano-technology Type I collagen Lumen (unit) Biomedical engineering |
| Zdroj: | Journal of Biomedical Materials Research Part A. 106:106-114 |
| ISSN: | 1549-3296 |
| Popis: | In vivo, tissues are drained of excess fluid and macromolecules by the lymphatic vascular system. How to engineer artificial lymphatics that can provide equivalent drainage in biomaterials remains an open question. The current study elucidates design principles for engineered lymphatics, by comparing the rates of removal of fluid and solute through type I collagen gels that contain lymphatic vessels or unseeded channels, or through gels without channels. Surprisingly, no difference was found between the fluid drainage rates for gels that contained vessels or bare channels. Moreover, solute drainage rates were greater in collagen gels that contained lymphatic vessels than in those that had bare channels. The enhancement of solute drainage by lymphatic endothelium was more pronounced in longer scaffolds and with smaller solutes. Whole-scaffold imaging revealed that endothelialization aided in solute drainage by impeding solute reflux into the gel without hindering solute entry into the vessel lumen. These results were reproduced by computational models of drainage with a flow-dependent endothelial hydraulic conductivity. This study shows that endothelialization of bare channels does not impede the drainage of fluid from collagen gels and can increase the drainage of macromolecules by preventing solute transport back into the scaffold. This article is protected by copyright. All rights reserved. |
| Databáze: | OpenAIRE |
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