Biotin biosynthesis: synthesis and biological evaluation of the putative intermediate thiols
| Autor: | Dominique Florentin, Michel Azoulay, Jean-Claude Tabet, Georges Guillerm, François Frappier, Andrée Marquet |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
chemistry.chemical_classification
biology Biotin synthase Biological activity General Chemistry biology.organism_classification medicine.disease_cause Biochemistry Bacillus sphaericus Enterobacteriaceae Catalysis chemistry.chemical_compound Colloid and Surface Chemistry chemistry Biotin Biosynthesis Thiol biology.protein medicine Escherichia coli |
| Zdroj: | Journal of the American Chemical Society. 115:2139-2145 |
| ISSN: | 1520-5126 0002-7863 |
| Popis: | Previous results led to the conclusion that the last step of biotin biosynthesis, the sulfuur insertion into dethiobiotin should involve a thiol as intermediate. The three possible thiols have been synthesized and their biological activity was evaluated: the primary thiol 10 labeled with [ 35 S] or [ 34 S] or with deuterium was transformed into biotin by resting cells of Bacillus sphaericus without loss of the label. Hence, it is a possible intermediate. Interestingly, growing cells transformed [ 35 S]- or 5-[ 2 H 2 ]-labeled 10 into biotin with loss of the labels |
| Databáze: | OpenAIRE |
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