High-Quality Genome Resource of Fusarium pseudograminearum Isolate Fp22-2 by Oxford Nanopore Long-Read Sequencing
| Autor: | Jia Tai, Xin Zhang, Xinlong Gao, Huiquan Liu, Ming Xu |
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| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Plant Disease. |
| ISSN: | 1943-7692 0191-2917 |
| Popis: | Fusarium crown rot (FCR), caused by Fusarium pseudograminearum, results in severe yield and quality losses of cereal crops in many arid and semi-arid areas of the world. Limited information about the genome of F. pseudograminearum restricts the pathogenesis research and breeding of disease-resistant wheat varieties. In this study, a high-quality genome assembly of F. pseudograminearum isolate Fp22-2 was generated using Oxford Nanopore long-read sequencing technology. The assembled nuclear genome of Fp22-2 is 37.33 Mb with a repeat content of 3.69%, divided into 4 contigs with a k-mer completeness score of 97.2% and a base quality accuracy of > 99.99%. A total of 14,533 protein-coding genes (BUSCO completeness score, 99.9%) were predicted and functionally annotated. Moreover, genes encoding pathogenic proteins including effector proteins and carbohydrate-active enzymes, and secondary metabolic gene clusters were identified. Overall, the high-quality genome assembly and gene annotation provided here will allow further investigating of the biology of F. pseudograminearum and lead to the development of new control options for FCR. |
| Databáze: | OpenAIRE |
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