Transcriptional Landscape in Epididymal White Adipose Tissue and Dorsal Root Ganglia in Obese Male Mice Exposed to Early Life Stress

Autor: Carolina Dalmasso, Jacqueline Leachman, Nermin Ahmed, Sundus Ghuneim, Eric Blalock, Analia Loria
Rok vydání: 2023
Předmět:
Zdroj: Physiology. 38
ISSN: 1548-9221
1548-9213
DOI: 10.1152/physiol.2023.38.s1.5732926
Popis: We have shown that male mice exposed to maternal separation and early weaning (MSEW), a mouse model of early life stress, display increased blood pressure and sympathetic activation compared to controls (C) when fed a high fat diet (HF). Obese MSEW males also show exacerbated pressor response to the acute stimulation of epididymal white adipose tissue (eWAT) with capsaicin, a transient receptor potential cation channel subfamily V member 1 (TrpV1) ligand. Further, selective afferent denervation reduces blood pressure in MSEW mice only. We also reported that increases in serotonin (5-HT) levels in eWAT could serve as a potential endogenous factor stimulating sensory neurons. The goal of this study was to identify whether MSEW induces changes in the transcriptomes of eWAT and dorsal root ganglia (DRG) that could be associated with sensory neuron stimulation. In addition, we investigated MSEW-induced changes in lipid class and kinases activity. After weaning, C57BL/6J male C and MSEW pups (n=4/group) were placed on HF (60% Kcal from fat) for 16 weeks. Then, mice were euthanized and eWAT and DRGs were collected for tissue analysis. Pamstation12 instrument (PamGene International, Netherlands) was used for kinase activity profiles. For lipidomic analysis, lipids were extracted using acidified organic solvents, and dry residues were reconstituted for analysis using a Shimadzu Nexera UHPLC system coupled with an AB Sciex 6500+ Q-Trap linear ion trap/triple quadrupole mass spectrometer. RNA-sequencing was performed by Novogene Corp Inc.Overall, MSEW decreased the phosphorylation of ~20 kinases in eWAT (e.g. AKT, SGK, PKA, PKG, JNK, PAKA, ERK, AMPK), while increasing phosphatidylglycerols (PG) in eWAT (p HL135158 This is the full abstract presented at the American Physiology Summit 2023 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.
Databáze: OpenAIRE
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