Development and Validation of a Quantitative Gas Chromatography ? Mass Spectrometry Confirmatory Method for Phenylbutazone in Equine Plasma
| Autor: | Phil Teale, S. Hines, Clive M. Pearce, J. Bright |
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| Rok vydání: | 2004 |
| Předmět: |
Chromatography
Equine plasma Chemistry Organic Chemistry Clinical Biochemistry Mass spectrometry Biochemistry Analytical Chemistry Prostaglandin-Endoperoxide Synthase Phenylbutazone medicine media_common.cataloged_instance Selected ion monitoring Gas chromatography–mass spectrometry European union Quantitative analysis (chemistry) medicine.drug media_common |
| Zdroj: | Chromatographia. 59:S109-S114 |
| ISSN: | 1612-1112 0009-5893 |
| DOI: | 10.1365/s10337-004-0243-7 |
| Popis: | The non-steroidal anti-inflammatory drug phenylbutazone is known to have a number of adverse side effects, however, its use in the equine remains widespread. To protect the human consumer, an action level of 50 ng mL−1 in plasma has been agreed by the European Union and regulatory compliance requires a methodology validated to the EU guidelines SANCO/1805/200 revision 6. Accordingly, a GC-MS method has been developed and validated to serve this purpose. The method counters the adverse effects of strong protein binding and the instability of phenylbutazone at low pH. Selected ion monitoring (SIM) allows the acquisition of three diagnostic ions and the use of a deuterated internal standard secures good quantitative performance. |
| Databáze: | OpenAIRE |
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