Biomechanics of red blood cells in human spleen and consequences for physiology and disease
| Autor: | George Em Karniadakis, Subra Suresh, Igor V. Pivkin, Zhangli Peng, Pierre Buffet, Ming Dao |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Multidisciplinary biology Thalassemia Spherocytosis hemic and immune systems Spleen Plasmodium falciparum 02 engineering and technology 021001 nanoscience & nanotechnology biology.organism_classification medicine.disease Microcirculation Hereditary spherocytosis Cell biology 03 medical and health sciences 030104 developmental biology medicine.anatomical_structure In vivo Immunology Circulatory system medicine 0210 nano-technology circulatory and respiratory physiology |
| Zdroj: | Proceedings of the National Academy of Sciences. 113:7804-7809 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.1606751113 |
| Popis: | Red blood cells (RBCs) can be cleared from circulation when alterations in their size, shape, and deformability are detected. This function is modulated by the spleen-specific structure of the interendothelial slit (IES). Here, we present a unique physiological framework for development of prognostic markers in RBC diseases by quantifying biophysical limits for RBCs to pass through the IES, using computational simulations based on dissipative particle dynamics. The results show that the spleen selects RBCs for continued circulation based on their geometry, consistent with prior in vivo observations. A companion analysis provides critical bounds relating surface area and volume for healthy RBCs beyond which the RBCs fail the “physical fitness test” to pass through the IES, supporting independent experiments. Our results suggest that the spleen plays an important role in determining distributions of size and shape of healthy RBCs. Because mechanical retention of infected RBC impacts malaria pathogenesis, we studied key biophysical parameters for RBCs infected with Plasmodium falciparum as they cross the IES. In agreement with experimental results, surface area loss of an infected RBC is found to be a more important determinant of splenic retention than its membrane stiffness. The simulations provide insights into the effects of pressure gradient across the IES on RBC retention. By providing quantitative biophysical limits for RBCs to pass through the IES, the narrowest circulatory bottleneck in the spleen, our results offer a broad approach for developing quantitative markers for diseases such as hereditary spherocytosis, thalassemia, and malaria. |
| Databáze: | OpenAIRE |
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