Development of poly(lactide-co-glycolide) nanoparticles functionalized with a mitochondria penetrating peptide
| Autor: | Silvia Marchianò, G. Magri, Sara Pellegrino, Francesca Selmin, Nicola Ferri, Chiara G.M. Gennari |
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| Rok vydání: | 2017 |
| Předmět: |
Circular dichroism
Cell Peptide 02 engineering and technology 010402 general chemistry 01 natural sciences Biochemistry chemistry.chemical_compound Structural Biology Drug Discovery medicine MTT assay Cytotoxicity Molecular Biology Pharmacology chemistry.chemical_classification Organic Chemistry General Medicine 021001 nanoscience & nanotechnology 0104 chemical sciences PLGA medicine.anatomical_structure chemistry Biophysics Molecular Medicine 0210 nano-technology Drug carrier Conjugate |
| Zdroj: | Journal of Peptide Science. 23:182-188 |
| ISSN: | 1075-2617 |
| DOI: | 10.1002/psc.2952 |
| Popis: | The development of mitochondria-targeting cell permeable vectors represents a promising therapeutic approach for several diseases, such as cancer and oxidative pathologies. Nevertheless, access to mitochondria can be difficult. A new hybrid material composed by poly(lactide-co-glycolide) (PLGA) functionalized with a 6-mer mitochondria penetrating peptide (MPP), consisting in alternating arginine and unnatural cyclohexylalanine, was developed. Circular dichroism, FT-IR and DSC studies indicated that the conjugation of the peptide with the polymer led to the obtainment of a more rigid material with respect to both PLGA and MPP as such. In particular, a conformational rearrangement to a helical structure was observed for MPP. MPP-PLGA conjugates were used for the preparation of nanoparticles that showed no cytotoxicity in MTT assay, suggesting their putative use for future studies on mitochondria targeting. Copyright © 2017 European Peptide Society and John Wiley & Sons, Ltd. |
| Databáze: | OpenAIRE |
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