Exo-β-1,3-Glucanase from Yeast InhibitsColletotrichum lupiniandBotrytis cinereaSpore Germination
| Autor: | David Kenneth Berger, Ian A. Dubery, Dean Oelofse |
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| Rok vydání: | 2009 |
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| Zdroj: | Journal of Phytopathology. 157:1-6 |
| ISSN: | 1439-0434 0931-1785 |
| DOI: | 10.1111/j.1439-0434.2008.01434.x |
| Popis: | Yeast exo-β-1,3-glucanase (EXG1) was evaluated as an inhibitory agent of Colletotrichum lupini and Botrytis cinerea. Extracts obtained from yeast transformed with the exgl gene, expressing high levels of EXG1 activity, or control untransformed yeast cultures that lacked EXG1 activity, were added to different starting concentrations of C. lupini fungal spore suspensions (2.5 × 10 3 to 80 × 10 3 spores per flask), and mycelial dry weight was measured after 5 days. Inhibition of C. lupini mycelial growth by EXG1 compared with control extracts ranged from 41 to 20% when added to starting fungal spore concentrations of 2.5 x 103 to 80 x 103, respectively. EXG1 activity in the extracts from the transformed yeast remained high over the 5-day incubation period. Addition of the EXG1 extract after C. lupini spore germination resulted in lower inhibition, indicating that the EXG1 targets the β-glucan in the cell walls of the fungal spores at an early stage of germination. Furthermore, the yeast EXG1 extracts were also shown to inhibit Botrytis cinerea spore germination and growth. Thus, the use of the yeast exgl gene for protection of crops, such as lupin and pear in transgenic strategies against C. lupini and B. cinerea, respectively, could be considered. |
| Databáze: | OpenAIRE |
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