Parasitological and molecular detection of bovine babesiosis in endemic areas of Karnataka state

Autor:	K U VIJAYAKUMAR, G C PUTTALAKSHMAMMA, PLACID E D'SOUZA, P P SENGUPTA, B M CHANDRANAIK, C RENUKAPRASAD
Rok vydání:	2017
Předmět:	General Veterinary Animal Science and Zoology
Zdroj:	The Indian Journal of Animal Sciences. 87
ISSN:	2394-3327 0367-8318
Popis:	A study was conducted to compare the specificity and sensitivity of parasitological methods (Giemsa and Fluorescent acridine orange staining) and PCR to detect the carrier status, subclinical and clinical babesiosis in cattle from endemic regions of Karnataka state in South India. Out of 148 cattle screened, 3 (2.03%), 7 (4.73%) and 16 (10.81%) were found positive for B. bigemina infection by Giemsa's staining, acridine orange staining and PCR, respectively. The conserved primers for B. bigemina derived from SpeI-AvaI restriction fragment were used in PCR which amplified a fragment of 278 bp in the positive cattle blood and was 83.26% sensitive and 91.16% specific for detection of babesiosis in cattle.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::2152b5b71c85f8069265d0e1f8fe2e52 https://doi.org/10.56093/ijans.v87i4.69512 Zobrazit plný text záznamu