Insulin-dependent and insulin-independent low Km cyclic AMP phosphodiesterase from rat adipose tissue
| Autor: | M M Appleman, H W Weber |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
chemistry.chemical_classification
medicine.medical_specialty Insulin medicine.medical_treatment Phosphodiesterase Adipose tissue Cell Biology Biology Epididymal fat Biochemistry Cyclic gmp Endocrinology chemistry Internal medicine medicine Nucleotide Insulin dependent Molecular Biology Homogenization (biology) |
| Zdroj: | Journal of Biological Chemistry. 257:5339-5341 |
| ISSN: | 0021-9258 |
| DOI: | 10.1016/s0021-9258(19)83780-8 |
| Popis: | Chromatographic analysis of a soluble extract of rat adipose tissue on DEAE-Sephacel resolves four distinct peaks of 3':5'-nucleotide phosphodiesterase (EC 3.1.4.17) activity. Kinetic investigation indicates that two of these fractions have a high affinity for cyclic AMP and show negative cooperative kinetic behavior at high substrate concentration. They differ in the degree of inhibition by cyclic GMP and in their response to insulin. If rat epididymal fat pads are incubated with insulin prior to homogenization, only one of the low Km cyclic AMP phosphodiesterase forms is stimulated. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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