Urea Biosensors Based on PVC Membrane Ion-Selective Electrodes

Autor:	Ewa Leszczyńska, Bożena Salamonowicz, Izabela Wałcerz, Stanisław Głąb, Robert Koncki
Rok vydání:	1996
Předmět:	Immobilized enzyme Urease biology Biochemistry (medical) Clinical Biochemistry Inorganic chemistry Enzyme electrode Biochemistry Analytical Chemistry Ion selective electrode chemistry.chemical_compound Membrane chemistry Electrochemistry biology.protein Carbonate Ion Ammonium Biosensor Spectroscopy
Zdroj:	Analytical Letters. 29:1939-1953
ISSN:	1532-236X 0003-2719
DOI:	10.1080/00032719608000800
Popis:	This paper presents a general method of enzyme immobilization at the surface of ion selective membranes. Covalent binding of enzymes directly on the electrode surface is a very effective method that results in stable enzymatic membranes. As an example the construction of enzymatic sensors for urea determination based on ammonium and hydrogen carbonate ion selective electrodes is presented. The optimum working conditions for these biosensors were found. Bioelectrodes based on an ammonium sensor show very good analytical parameters: dynamic stability - over 2 months without decrease of sensitivity, response time - shorter then 20 s. high sensitivity, determination range from 0.3 to 70 mM. In the contrast to the ammonium ion based biosensors, those constructed on the basis of anion selective electrodes have worse analytical parameters. It is mainly due to poor selectivity and instability of an applied ion selective electrode. In spite of this, both types of urea biosensors were used for measurements...
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::2145e121e85d3682bb6f7d08757fc215 https://doi.org/10.1080/00032719608000800 Zobrazit plný text záznamu