Assessment of genetic diversity of yellow-seeded rapeseed (Brassica napus L.) accessions by AFLP markers
| Autor: | Ovesna, J.,Vyzkumny Ustav Rostlinne Vyroby, Prague, Dotlacil, L.,Vyzkumny Ustav Rostlinne Vyroby, Prague, Yu, C.,Northwest A, Kucera, V.,Vyzkumny Ustav Rostlinne Vyroby, Prague, Hu, S.,Northwest A, Vyvadilova, M.,Vyzkumny Ustav Rostlinne Vyroby, Prague, Leisova, L.,Vyzkumny Ustav Rostlinne Vyroby, Prague |
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| Rok vydání: | 2007 |
| Předmět: |
0301 basic medicine
Genetics Genetic diversity Rapeseed biology Pedigree information Brassica UPGMA food and beverages Plant Science biology.organism_classification 03 medical and health sciences Horticulture 030104 developmental biology Genetic marker Multiplex polymerase chain reaction Amplified fragment length polymorphism |
| Zdroj: | Czech Journal of Genetics and Plant Breeding. 43:105-112 |
| ISSN: | 1805-9325 1212-1975 |
| DOI: | 10.17221/2071-cjgpb |
| Popis: | The genetic diversity of 35 yellow-seeded Brassica napus L. accessions originating from China, Czech Republic and Poland was assessed by means of Amplified Fragment Length Polymorphism (AFLP) markers based on multiplex PCR using multi-colour fluorescent-labelled primers. Five brown-seeded accessions originating from China and France were selected as outliers. In total, 632 peaks were generated by AFLP reaction using 18 primer combinations. Only distinctly polymorphic markers among them were scored. In total, 242 polymorphic mark - ers were detected with an average of 13.4 markers per primer combination. The AFLP analysis separated forty studied accessions into Chinese and European groups by UPGMA clustering and Principal Coordinates Analysis (PCA). The grouping of accessions based on the cluster analysis and PCA was generally consistent with known pedigree information and geographic origin. Notable geographical divergence was found between Chinese and European yellow-seeded accessions. This information is useful for yellow-seeded hybrid breeding and encourag |
| Databáze: | OpenAIRE |
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