Investigation of the Mg-HCO3−-ATPase activity of thyroid tissue cells under various pathologies

Autor:	Zurab Kuchukashvili, K. O. Menabde, Nana Koshoridze
Rok vydání:	2012
Předmět:	chemistry.chemical_classification medicine.medical_specialty Adenoma Endoplasmic reticulum ATPase Clinical Biochemistry Thyroid General Medicine Biology Mitochondrion medicine.disease Enzyme assay Enzyme Endocrinology medicine.anatomical_structure chemistry Internal medicine medicine biology.protein Cell fractionation
Zdroj:	Scandinavian Journal of Clinical and Laboratory Investigation. 72:363-368
ISSN:	1502-7686 0036-5513
Popis:	Distribution of the Mg²⁺-dependent, HCO₃⁻-stimulated ATPase (HCO₃⁻-ATPase) was investigated in sub-cellular fractions of the tissue of the human thyroid gland. It was found that especially high enzymatic activity is characteristic of mitochondria and the endoplasmic reticulum. Correlation between various pathologies of the thyroid and HCO₃⁻-ATPase activity was demonstrated. The activity was evaluated using the difference between active and passive ATPase. During development of the various pathologies, the sub-cellular fractions of the gland showed uneven alterations of HCO₃⁻-ATPase activity. Intriguingly, the enzyme kinetic parameters are also changed, i.e. in the different pathologies both enzyme activity and its affinity towards bicarbonate ions are altered. This raises the question whether pathology is caused by, or results in, changes in the enzyme at the molecular level.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::2142f87fe506dee187490d25e1b5cf2f https://doi.org/10.3109/00365513.2012.675442 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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