Spontaneous aggregation of locust lipophorin during hemolymph collection

Autor:	Haruo Chino, Roger G.H. Downer, Yukari Kiyomoto, Yuji Hirayama, Koui Takahashi
Rok vydání:	1987
Předmět:	Gel electrophoresis animal structures biology biology.organism_classification Biochemistry Acrididae Gel permeation chromatography Insect Science Hemolymph Ultracentrifuge Adipokinetic hormone Molecular Biology Polyacrylamide gel electrophoresis Locust
Zdroj:	Insect Biochemistry. 17:89-97
ISSN:	0020-1790
DOI:	10.1016/0020-1790(87)90148-x
Popis:	Density-gradient ultracentrifugation, gel permeation chromatography, polyacrylamide gel electrophoresis and electron microscopy were used to provide an experimental basis for the proposal that locust lipophorin aggregates spontaneously during the collection of hemolymph through a puncture in the neck or coxal region of the insect. The evidence presented in this paper supports the above proposal and clearly demonstrates that the “puncture” method promotes the aggregation of lipophorin molecules and results in the formation of large aggregates as an artificial product. Thus, the use of this method should be discouraged for the study of lipophorin in locusts.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::213e98ac8f1e36199f02a3e770e8999c https://doi.org/10.1016/0020-1790(87)90148-x Zobrazit plný text záznamu