Dengue virus induced changes in Ca 2+ homeostasis in human hepatic cells that favor the viral replicative cycle
| Autor: | Martha Yocupicio-Monroy, Carlos Vazquez, Romel Rosales, José L. Zambrano, Juan E. Ludert, Marie Christine Ruiz, Franshelle Peña, Rosa M. del Angel, Luis A. Constantino-Jonapa, Cinthia L. Dionicio |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cancer Research viruses Endoplasmic reticulum virus diseases STIM1 Dengue virus Biology medicine.disease_cause biology.organism_classification Cell biology 03 medical and health sciences 030104 developmental biology Infectious Diseases Viral replication Cell culture Vesicular stomatitis virus Virology Extracellular medicine Homeostasis |
| Zdroj: | Virus Research. 245:17-28 |
| ISSN: | 0168-1702 |
| DOI: | 10.1016/j.virusres.2017.11.029 |
| Popis: | The role of Ca2+ during dengue virus (DENV) replication is unknown; thus, changes in Ca2+ homeostasis in DENV infected human hepatic HepG2 and Huh-7 cells were analyzed. Infected HepG2 cells, but not Huh-7 cells, showed a significant increase in plasma membrane permeability to Ca2+, while both cell lines showed marked reduced levels of Ca2+ stored in the endoplasmic reticulum. While the expression levels of STIM1 and ORAI1 showed no changes, STIM1 and ORAI1 were shown to co-localized in infected cells, indicating activation of the store-operated Ca2+ entry (SOCE) pathway. Finally, manipulation in the infected cells of the intra and extracellular Ca2+ levels by chelators (BAPTA-AM and EGTA), SOC inhibitor (SKF96365), IP3 Receptor antagonist (2APB) or increase of extracellular [Ca2+], significantly reduced DENV yield, but not vesicular stomatitis virus yield, used as a control. These results show that DENV infection alters cell Ca2+ homeostasis and that such changes favor viral replication. |
| Databáze: | OpenAIRE |
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