Inhibitory effects of a collagen–mineral complex on deterioration of bone mass and bone quality in ovariectomized mice

Autor: Hisashi Innami, Ryota Kawamata, Takashi Sakurai, Hiromi Wakao, Yusuke Kozai, Mikiko Iino
Rok vydání: 2013
Předmět:
Zdroj: Oral Radiology. 30:203-211
ISSN: 1613-9674
0911-6028
DOI: 10.1007/s11282-013-0160-2
Popis: To characterize the osteoporosis-preventive effects of a novel material consisting of a complex of type 1 collagen and hydroxyapatite (CCH) in osteoporosis model mice. Ten-week-old ovariectomized (OVX) ICR mice were used. The five study groups comprised a sham group, OVX control group, calcium carbonate (CaCO3) group, vitamin K2 [menaquinone 7 (MK7)] group, and CHH group (n = 10 each). The CaCO3 and CHH groups were fed a special diet containing 2 % of the respective substance, whereas the MK7 group received a special diet containing 0.5 % MK7. After maintenance of the animals under these conditions for 8 weeks, their femurs were removed and blood samples were collected. The bone mineral densities (BMDs) of the distal femoral epiphysis were analyzed by peripheral quantitative computed tomography. Three-dimensional images were rendered with micro-focus computed tomography, and the trabecular structures were determined. The significantly lower BMDs in the epiphyseal and diaphyseal regions of the OVX control group femurs compared with the sham group femurs were clear osteoporotic signs. The trabecular structure analyses revealed 12 parameters that were significantly lower in the OVX control group compared with the sham group. No distinct changes in the BMD or trabecular structure were observed in the CaCO3 group. Significant improvements in the BMD in the MK7 group were limited to three parameters, whereas 11 trabecular structure parameters were significantly improved. The CHH group showed significant improvements in seven BMD parameters and seven trabecular structure parameters. CHH improves trabecular structure and BMD in a well-balanced manner.
Databáze: OpenAIRE
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