Enzyme-linked immunoassay for the detection of glyphosate in food samples using avian antibodies

Autor:	M. A. Sreenivasa, H. K. Manonmani, A. Arul Selvi
Rok vydání:	2011
Předmět:	chemistry.chemical_classification Detection limit Chromatography Immunology Pesticide High-performance liquid chromatography chemistry.chemical_compound Enzyme chemistry Glyphosate Reagent Agronomy and Crop Science Hapten Food Science Conjugate
Zdroj:	Food and Agricultural Immunology. 22:217-228
ISSN:	1465-3443 0954-0105
DOI:	10.1080/09540105.2011.553799
Popis:	A simple competitive immunoassay was developed for the measurement of glyphosate in food samples. It employed the avian antibodies (IgY) that recognised glyphosate as a capture reagent and glyphosate–alkaline phosphatase conjugate as an enzyme label. The assay depended on the competitive binding between the antibody and glyphosate derived from food samples for binding sites with immobilised glyphosate–OVA conjugate. The concentration of glyphosate in the food samples was quantified by the ability of the pesticide present in food samples to inhibit the binding of the enzyme conjugate to the antibody and subsequently the colour formation in the assay. The assay was specific to glyphosate with a limit of detection of 2 ppb. Mean analytical recovery of glyphosate in different food samples was 9.00–134.00%. The precision of the assay was satisfactory. The assay compared favourably with high-pressure liquid chromatography (HPLC) in its ability to accurately measure glyphosate in the food samples.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::212bf96f975277d127407c0755275dfa https://doi.org/10.1080/09540105.2011.553799 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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