Chromosomal assembly of the Antarctic toothfish (Dissostichus mawsoni) genome using third-generation DNA sequencing and Hi-C technology
Autor: | Euna Jo, E. Choi, Seok-Gwan Choi, Sangdeok Chung, Jeong-Hoon Kim, Hyun Park, Jinmu Kim, Hyun-Woo Kim, Seung Jae Lee |
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Rok vydání: | 2021 |
Předmět: |
0303 health sciences
Dissostichus Ecology biology Gene prediction 030302 biochemistry & molecular biology biology.organism_classification Genome DNA sequencing 03 medical and health sciences Evolutionary biology Nototheniidae Animal Science and Zoology Antarctic toothfish Gene Ecology Evolution Behavior and Systematics 030304 developmental biology Reference genome |
Zdroj: | Zoological Research. 42:124-129 |
ISSN: | 2095-8137 |
Popis: | The Antarctic toothfish, Dissostichus mawsoni, belongs to the Nototheniidae family and is distributed in sub-zero temperatures below S60° latitude in the Southern Ocean. Therefore, it is an attractive model species to study the stenothermal cold-adapted character state. In this study, we successfully generated highly contiguous genome sequences of D. mawsoni, which contained 1 062 scaffolds with a N50 length of 36.98 Mb and longest scaffold length of 46.82 Mb. Repetitive elements accounted for 40.87% of the genome. We also inferred 32 914 protein-coding genes using in silico gene prediction and transcriptome sequencing and detected splicing variants using Isoform-Sequencing (Iso-Seq), which will be invaluable resource for further exploration of the adaptation mechanisms of Antarctic toothfish. This new high-quality reference genome of D. mawsoni provides a fundamental resource for a deeper understanding of cold adaptation and conservation of species. |
Databáze: | OpenAIRE |
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