Protein kinase Cδ (PKCδ) involved in the regulation of pAkt1 (Ser473) on the release of mouse oocytes from diplotene arrest
| Autor: | Gensong Li, Chen Feng, Lingling Liu, Dahai Yu, Sen Li, Chunyu Li, Hanwen Li, Yu Cao, Xin Deng |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Meiotic spindle organization Somatic cell Chemistry Clinical Biochemistry AKT1 Cell Biology General Medicine Biochemistry Cell biology Chromosome segregation 03 medical and health sciences 030104 developmental biology Downregulation and upregulation Phosphorylation Protein kinase A Protein kinase B |
| Zdroj: | Cell Biochemistry and Function. 36:221-227 |
| ISSN: | 0263-6484 |
| DOI: | 10.1002/cbf.3334 |
| Popis: | Defects in meiotic maturation may lead to chromosome segregation errors and genomic instability. Previous reports indicated that protein kinase C delta (PKCδ) may interact with microtubule organizing center-associated protein to play a role on meiotic spindle organization of mammalian oocytes. In this study, we explored the potential role of protein kinase B alpha (PKBα/Akt1), PKCδ, and their relationship on meiotic maturation of mouse oocytes. We examined the expression and localization of pAkt1 (Ser473) and/or pPKCδ (Thr505) under the treatment of SH-6 or Sotrastaurin. With the increasing of SH-6 concentrations, we observed that the protein levels of pAkt1 (Ser473) and the percentages of GVBD were decreased gradually. And the distribution of pAkt1 (Ser473), Cdc25B, pCdc2 (Tyr15), and α-tubulin around nucleus was also highly disordered under the treatment of 10-μM SH-6, a special inhibitor of pAkt1 (Ser473). In addition, the levels of pAkt1 (Ser473) were decreased with the treatment of Sotrastaurin, an inhibitor of PKCδ, suggesting that Akt1 may be one of the downstream targets of PKCδ. So, we deduced that PKCδ may be involved in regulating the release from diplotene arrest of mouse oocytes by controlling the levels of pAkt1 Ser473. Significance of the study As a drug target, Akt is still the focus of research in somatic and fertilized eggs. Here, our data depicted a series of molecular events of pAkt1 (Ser473) and PKCδ via their special inhibitor and antibody in order to analyse their role and relationship on the release of mouse oocytes from diplotene arrest. Our results may offer important data for clinic research scientist to recognize the target role of pAkt1 (Ser473) via PKCδ in cell cycle regulation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text