Laboratory Accuracy Improvement in the UK NEQAS Leucocyte Immunophenotyping Immune Monitoring Program: An Eleven-Year Review via Longitudinal Mixed Effects Modeling
| Autor: | Wes Rountree, John Bainbridge, Raul Louzao, David Barnett, John Wong, Liam Whitby, Thomas N. Denny |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Histology Quality management business.industry Quality assessment Cell Biology Immune monitoring Accuracy improvement Pathology and Forensic Medicine Clinical trial 03 medical and health sciences 030104 developmental biology Immunophenotyping External quality assessment Mixed effects Medicine Medical physics business |
| Zdroj: | Cytometry Part B: Clinical Cytometry. 94:250-256 |
| ISSN: | 1552-4949 |
| Popis: | Background: The United Kingdom National External Quality Assessment Service (UK NEQAS) for Leucocyte Immunophenotyping Immune Monitoring Programme, provides external quality assessment (EQA) to non-U.S. laboratories affiliated with the NIH NIAID Division of AIDS (DAIDS) clinical trials networks. Selected laboratories are required to have oversight, performance monitoring and remediation undertaken by Immunology Quality Assessment (IQA) staff under the DAIDS contract. We examined whether laboratory accuracy improves with longer EQA participation and whether IQA remediation is effective. Methods: Laboratory accuracy, defined by the measurement residuals from trial sample medians, was measured on four outcomes: both CD4+ absolute counts (cells/μL) and percentages; and CD8+ absolute counts (cells/μL) and percentages. Three laboratory categories were defined: IQA monitored (n=116), United Kingdom/non-DAIDS (n=137), and non-DAIDS/non-UK (n=1034). For absolute count outcomes, the groups were subdivided into single platform (SPT) and dual platform (DPT) users. Results: Increasing EQA duration was found to be associated with increasing accuracy for all groups in all four lymphocyte subsets (p |
| Databáze: | OpenAIRE |
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