A simple and efficient preparative procedure for menaquinone-7 from Bacillus subtilis (natto) using two-stage extraction followed by microporous resins
| Autor: | Fang Zhiwei, Zhiming Zheng, Ni Wenfeng, Peng Wang, Hongfei Wei, Hui Liu, Wang Han, Genhai Zhao, Li Wang |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0303 health sciences Chromatography Ethanol biology Extraction (chemistry) Bioengineering Bacillus subtilis (natto) Microporous material Bacillus subtilis biology.organism_classification 01 natural sciences Applied Microbiology and Biotechnology Biochemistry law.invention 03 medical and health sciences chemistry.chemical_compound chemistry law 010608 biotechnology Yield (chemistry) Fermentation Crystallization 030304 developmental biology |
| Zdroj: | Process Biochemistry. 83:183-188 |
| ISSN: | 1359-5113 |
| DOI: | 10.1016/j.procbio.2019.05.008 |
| Popis: | Meanquinone-7 (MK-7) can be produced by the fermentation of Bacillus subtilis (natto), which is known for its nutraceutical and pharmacological properties. This study focused on the separation and purification of meanquinone-7 from wet cells produced by Bacillus subtilis (natto) fermentation. Using ethanol to extract menaquinone-7 twice directly from the crude cells after fermentation produced a menaquinone-7 yield of 1.47mg/g. The crude extract was purified using macroporous resin and subsequent crystallization. After treatment with HPD722 resin, the purification of menaquinone-7 were 7.17-fold increased with recovery yields of 97.2%. Menaquinone-7 with purities of more than 96% were produced with recovery yield of 99.3% by recrystallized with 95% ethanol. The developed procedure can produce high-purity of menaquinone-7 as well as high recoveries. This is an economical, efficient and simple process to large-scale preparation of high-purity menaquinone-7. |
| Databáze: | OpenAIRE |
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