Lectin-Gold Staining of Sialic Acid in Mesotheliomas and Pulmonary Adenocarcinomas

Autor:	Polly R. Schoning
Rok vydání:	1997
Předmět:	Pathology medicine.medical_specialty Histology biology Lectin law.invention Sialic acid Staining Medical Laboratory Technology chemistry.chemical_compound chemistry law Gold particles Lowicryl K4M biology.protein medicine Glutaraldehyde Anatomy Electron microscope Positive staining
Zdroj:	Journal of Histotechnology. 20:31-34
ISSN:	2046-0236 0147-8885
DOI:	10.1179/his.1997.20.1.31
Popis:	Lectin-gold staining and electron microscopy techniques were used to determine the sialic acid patterns of epithelial mesotheliomas and pulmonary adenocarcinomas. Pulmonary adenocarcinomas and 4 malignant epithelial mesotheliomas from human tissue banks were used. Tissues were fixed in a mixture of 3% parafomaldehyde and 0.1% glutaraldehyde for 2 hr, dehydrated in increasing concentrations of ethanol, and embedded in Lowicryl K4M. Ultrathin sections on 150-mesh nickel grids were incubated with LimaxJavus lectin-gold (LFL-gl0), Sambucus nigrans lectin-gold (SNL-gl4), and Maackia amurensis lectin-gold (MAL-gl4). Both mesotheliomas and adenocarcinomas stained positively with (LFL-gl0) and (MAL-g14) and negatively with (SNL-gl4). Positive staining with MAL-g14 and an absence of staining with SNL-g14 show the terminal sialic acid linkage to D-galactose to be α2,3, rather than α2,6. Although, mesotheliomas stained with MAL-g14 had significantly more gold particles than did adenocarcinomas, the gold par...
Databáze:	OpenAIRE
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