Optimized whole cell biocatalyst from acetoin to 2,3-butanediol through coexpression of acetoin reductase with NADH regeneration systems in engineeredBacillus subtilis
| Autor: | Tuyishime Philibert, Niyomukiza Samuel, Teng Bao, Yang Taowei, Li Xin, Zhiming Rao, Meijuan Xu, Xian Zhang, Irene Komera |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
biology Renewable Energy Sustainability and the Environment Bioconversion General Chemical Engineering Acetoin Organic Chemistry NADH regeneration Bacillus subtilis biology.organism_classification Formate dehydrogenase Pollution Inorganic Chemistry 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Fuel Technology chemistry Biochemistry Glucose dehydrogenase 2 3-Butanediol Fermentation Waste Management and Disposal Biotechnology |
| Zdroj: | Journal of Chemical Technology & Biotechnology. 92:2477-2487 |
| ISSN: | 0268-2575 |
| DOI: | 10.1002/jctb.5267 |
| Popis: | BACKGROUND 2,3-Butanediol (2,3-BD) has a wide range of applications in chiral molecular synthesis, biofuel additives, and in food flavor additive manufacturing. Fermentation is a favorable method for 2,3-BD production. However, it requires much time and produces several NADH related byproducts which compete with 2,3-BD production. Bacillus subtilis has an excellent ability for 2,3-BD production by biocatalysis. However, its production is limited by low intracellular NADH and the reversible property of acetoin reductase (AR/2,3-BDH). The whole cell biocatalyst process with two different NADH regeneration systems was designed for efficient production of 2,3-BD in B. subtilis 168. RESULTS Formate dehydrogenase and glucose dehydrogenase for NADH regeneration were successfully co-expressed with acetoin reductase in B. subtilis 168. After optimization of biocatalyst bioconversion conditions, B. subtilis 168/pMA5-bdhA-HpaII-fdh yielded 74.5 g L−1 of 2, 3-BD with 9.3 g L−1 h−1 productivity by fed batch and 115.4 g of 2,3-BD was achieved using same batch bacterium by three repeated batch bioconversions. On the other hand, 63.7 g L−1 of 2, 3-BD was produced with 7.92 g L−1 h−1 productivity by B. subtilis 168/pMA5-bdhA-HpaII-gdh. To our knowledge, the volume productivity obtained here is the highest ever reported for biocatalysis. CONCLUSION A higher productivity of 2,3-BD from acetoin was achieved by whole cell biocatalysis with NADH regeneration systems in B. subtilis 168. This approach can be applied for NADH related bio-based chemicals production to improve titer, yield and productivity. © 2017 Society of Chemical Industry |
| Databáze: | OpenAIRE |
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