An Improved Method for Studying the Enzyme-Catalyzed Oxidation of Glucose Using Luminescent Probes

Autor:	Matthew Cuber, William D. Bare, Chi V. Pham, James N. Demas
Rok vydání:	2007
Předmět:	Enzyme catalyzed biology Chemistry Inorganic chemistry Kinetics Analytical chemistry chemistry.chemical_element Improved method General Chemistry Fluorescence Oxygen Education biology.protein Glucose oxidase Enzyme kinetics Luminescence
Zdroj:	Journal of Chemical Education. 84:1511
ISSN:	1938-1328 0021-9584
Popis:	A new method is presented for measuring the rate of the oxidation of glucose in the presence of glucose oxidase. The improved method employs luminescence measurements to directly determine the concentration of oxygen in real time, thus obviating complicated reaction schemes employed in previous methods. Our method has been used to determine kinetic parameters including Vmax, Km, and kcat, all in good agreement with published values. The method can also be used to investigate specificity and inhibition. Information is included pertaining to the construction of a low-cost fluorescence instrument suitable for the measurements in this protocol.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::20eb983e65b4a365bce32dc82c4632d2 https://doi.org/10.1021/ed084p1511 Zobrazit plný text záznamu