HPLC Method Development and Validation of S(-)-Carvedilol from API and Formulations

Autor:	Chandupatla Vijitha, Ciddi Veeresham, Ettireddy Swetha
Rok vydání:	2015
Předmět:	Chiral column chromatography Active ingredient Psychiatry and Mental health Chromatography Chemistry medicine Particle size Uv detection Enantiomer Hplc method Carvedilol Chiral resolution medicine.drug
Zdroj:	American Journal of Analytical Chemistry. :437-445
ISSN:	2156-8278 2156-8251
DOI:	10.4236/ajac.2015.65043
Popis:	A simple chiral HPLC method was developed and validated for quantification of S(-)-Carvedilol in Active Pharmaceutical Ingredient (API) and marketed tablet formulation of racemic Carvedilol. Chiral resolution of enantiomers of Carvedilol was achieved on Phenomenex Lux-cellulose–4 (250 mm × 4.6 mm; 5 μ particle size) chiral column by using a mobile phase, Isopropanol and n-Heptane (60:40 v/v), at a flow rate of 1.0 ml/min and by employing UV detection at 254 nm wavelength. The method was validated according to the ICH guidelines and was proved to be specific, linear, precise and accurate for the analysis of S(-)-Carvedilol.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::20e6681bc8c622293369df510c93d890 https://doi.org/10.4236/ajac.2015.65043 Zobrazit plný text záznamu