Characterization of a novel marine aerobic denitrifier Vibrio spp. AD2 for efficient nitrate reduction without nitrite accumulation

Autor: Yunjie Ruan, Yinghai Wu, Jilong Ren, Chenzheng Wei, Liu Ying, Jing Song, Hongjing Ma, Han Rui
Rok vydání: 2021
Předmět:
Zdroj: Environmental Science and Pollution Research. 28:30807-30820
ISSN: 1614-7499
0944-1344
DOI: 10.1007/s11356-021-12673-8
Popis: Aerobic denitrifiers have the potential to reduce nitrate in polluted water under aerobic conditions. A salt-tolerant aerobic denitrifier was newly isolated and identified as Vibrio spp. AD2 from a marine recirculating aquaculture system, in which denitrification performance was investigated via single-factor experiment, Box–Behnken experiment, and nitrogen balance analysis. Nitrate reductase genes were identified by polymerase chain reaction. Results showed that strain AD2 removed 98.9% of nitrate-nitrogen (NO3−-N) with an initial concentration about 100 mg·L−1 in 48 h without nitrite-nitrogen (NO2−-N) accumulation. Nitrogen balance indicated that approximately 17.5% of the initial NO3−-N was utilized for bacteria synthesis themselves, 4.02% was converted to organic nitrogen, 39.8% was converted to nitrous oxide (N2O), and 31.1% was converted to nitrogen (N2). Response surface methodology experiment showed that the maximum removal of total nitrogen (TN) occurred under the condition of C/N ratio 11.5, shaking speed 127.9 rpm, and temperature 30.8 °C. Sequence amplification indicated that the denitrification genes, napA and nirS, were present in strain AD2. These results indicated that the strain AD2 has potential applications for removing NO3−-N from high-salinity (3%) wastewater.
Databáze: OpenAIRE
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