| Autor: | Betty Heremans, Michel Jacobs |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
endocrine system diseases
biology Mutant Lysine nutritional and metabolic diseases Mutagenesis (molecular biology technique) General Medicine biology.organism_classification complex mixtures Biochemistry Isozyme Arabidopsis Genetics bacteria Arabidopsis thaliana Aspartate kinase Threonine Molecular Biology hormones hormone substitutes and hormone antagonists Ecology Evolution Behavior and Systematics |
| Zdroj: | Biochemical Genetics. 35:139-153 |
| ISSN: | 0006-2928 |
| DOI: | 10.1023/a:1022265825402 |
| Popis: | Mutagenesis and subsequent selection of Arabidopsis thaliana plantlets on a growth inhibitory concentration of lysine has led to the isolation of lysine-resistant mutants. The ability to grown on 2 mM lysine has been used to isolate mutants that may contain an aspartate kinase with altered regulatory-feedback properties. One of these mutants (RL 4) was characterized by a relative enhancement of soluble lysine. The recessive monogenic nuclear transmission of the resistance trait was established. It was associated with an aspartate kinase less sensitive to feedback inhibition by threonine. Two mutants (RLT 40 and RL 4) in Arabidopsis, characterized by an altered regulation of aspartate kinase, were crossed to assess the effects of the simultaneous presence of these different aspartate kinase forms. A double mutant (RLT40 x RL4) was isolated and characterized by two feedback-desensitized isozymes of aspartate kinase to, respectively, lysine and threonine but no threonine and/or lysine overproduction was observed. Genetical analysis of this unique double aspartate kinase mutant indicated that both mutations were located on chromosome 2, but their loci (ak1 and ak2) were found to be unlinked. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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