Mutagenicity of 8-ethoxycaffeine in vitro
| Autor: | Renate Strobel, G. Röhrborn, Gabriele Roszinsky-Köcher |
|---|---|
| Rok vydání: | 1979 |
| Předmět: |
Genetics
Salmonella endocrine system diseases Health Toxicology and Mutagenesis Point mutation Chinese hamster ovary cell Biology medicine.disease_cause Molecular biology female genital diseases and pregnancy complications In vitro S9 fraction medicine Microsome Sister chromatids Molecular Biology Incubation |
| Zdroj: | Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. 60:349-355 |
| ISSN: | 0027-5107 |
| DOI: | 10.1016/0027-5107(79)90025-3 |
| Popis: | The caffeine derivative 8-ethoxycaffeine (EOC) was tested in 3 different test systems in vitro. Each experiment was carried out with and without S9 mix. Incubation temperatures were 20 and 37dgC. 1. (1) In the Salmonella/microsome test, EOC behaved as a pro-mutagen in the Salmonella typhimurium strain TA1535. No mutagenic activity was found in experiments without S9 mix. The influence of temperature was negligible. The mutagenic activity of EOC depended mainly on the mammals used to prepare the S9 fraction and on the agents given to them to induce liver enzymes. 2. (2) EOC did not induce sister-chromatid exchanges in cell cultures, either at 20 or at 37°C. 3. (3) On the other hand, EOC induced chromosomal aberrations when the cells were incubated at 37°C without S9 mix. |
| Databáze: | OpenAIRE |
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