Inhibition of Tumor Metastasis by a Synthetic Polymer Containing a Cell-Adhesive RGDS Peptide
| Autor: | Atsushi Orikasa, Masayoshi Kojima, Hiroyuki Komazawa, Mitsunori Ono, Isamu Itoh, Ikuo Saiki, Yu Igarashi, Ichiro Azuma |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
chemistry.chemical_classification
Matrigel Polymers and Plastics Tetrapeptide Cell adhesion molecule Chemistry Melanoma 0206 medical engineering Cell Bioengineering Peptide 02 engineering and technology 021001 nanoscience & nanotechnology medicine.disease 020601 biomedical engineering Molecular biology Metastasis Biomaterials medicine.anatomical_structure Biochemistry embryonic structures Materials Chemistry medicine 0210 nano-technology Conjugate |
| Zdroj: | Journal of Bioactive and Compatible Polymers. 8:258-274 |
| ISSN: | 1530-8030 0883-9115 |
| DOI: | 10.1177/088391159300800305 |
| Popis: | A water soluble polymer containing cell adhesive Arg-Gly-Asp- Ser (RGDS) sequence, i.e., poly(carboxyethylmethacrylamide-RGDS) conjugate [poly(CEMA-RGDS)], was synthesized. Poly(CEMA-RGDS) inhibited lung metastasis of B16-BL6 melanoma cells in a dose-dependent manner (20-1000 μg) and liver metastasis of L5178YML25 lymphoma cells. A mixture of poly- (CEMA) and RGDS peptide or poly(CEMA) alone did not show any inhibitory effect on lung metastasis. The Gly-Arg-Gly-Asp-Ser (GRGDS) peptide required high doses (3000 μ g) to obtain a sufficient antimetastatic effect. An in vitro study showed that poly(CEMA-RGDS) as well as RGDS + poly(CEMA) gave similar inhibition of B16-BL6 cell invasion into reconstituted basement mem brane Matrigel. Intermittent i.v. administration of poly(CEMA-RGDS) after in oculation with B16-BL6 cells caused significant inhibition of spontaneous lung metastasis as compared with the multiple administration of RGDS, poly- (CEMA) or untreated control. These results demonstrate that the conjugation |
| Databáze: | OpenAIRE |
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