| Autor: | M Florent, Jean Jacques Ballet, Pascal Gauduchon, Brigitte Sola, T. Godard |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
0303 health sciences
Programmed cell death Health Toxicology and Mutagenesis Growth factor medicine.medical_treatment Cell Biology Phosphatidylserine Biology Toxicology Molecular biology Cell biology Comet assay 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine chemistry Apoptosis Annexin 030220 oncology & carcinogenesis medicine DNA fragmentation Fragmentation (cell biology) 030304 developmental biology |
| Zdroj: | Cell Biology and Toxicology. 15:185-192 |
| ISSN: | 0742-2091 |
| DOI: | 10.1023/a:1007641821779 |
| Popis: | Dysregulation of apoptosis contributes to various diseases such as neurodegenerative or aging disorders, autoimmune syndromes or cancers. Numerous experimental paradigms have been explored to characterize molecular and cellular modulators of apoptosis. Similarly, numerous techniques have been described for detecting and/or quantifying accurately cells committed to apoptosis. Besides the conventional techniques, we describe in this report that the comet assay, which detects DNA single- and double-strand breaks in situ, at the cellular level, is relevant for the characterization of apoptotic cells. The comet assay is very sensitive and detects DNA fragmentation occurring in the apoptotic process as early as exposure of phosphatidylserine residues on the outer leaflet. Thus the comet assay can be used for the recognition of apoptosis that follows the death signal caused, for example, by genotoxic stress as well as lack of survival signal as in growth factor deprivation. |
| Databáze: | OpenAIRE |
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