721 INOSINE IMPROVES BLADDER FUNCTION IN RATS WITH SPINAL CORD INJURY
| Autor: | Maryrose P. Sullivan, Aruna Ramachandran, Yeun Goo Chung, Carlos R. Estrada, Eric Boxer, Dolores Di Vizio, Lin Huang, Duong Tu, Vivian Cristofaro, Rosalyn M. Adam, Abhishek Seth |
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| Rok vydání: | 2012 |
| Předmět: | |
| Zdroj: | Journal of Urology. 187 |
| ISSN: | 1527-3792 0022-5347 |
| Popis: | intensities analysed on Image J. Two or one-factor anovas were utilised as applicable, with post-hoc t-tests. RESULTS: DNC increased localization of DNMT3A to the nucleus of BSMC, in contrast to NC cells, which expressed only cytoplasmic DNMT3A. The JAK2/STAT inhibitor AG490 significantly reduced DNMT3A nuclear localization (P 0.001), without changing SMA expression and proliferation compared to control. Hypoxia enhanced DNMT3A and 3B localization to the nucleus on DNC, but had no effect on NC. Aza prevented DNC-induced proliferation (p 0.05). On DNC, Sonic hedgehog (SHH) upregulated expression of SMA, but SHH increased absolute levels of both nuclear and cytoplasmic DNMT3A (p 0.05). CONCLUSIONS: Matrix exquisitely regulates DNMT3A localization and expression, and influences differentiation in BSMCs exposed to denatured matrix /growth factors or SHH. That nuclear expression of DNMT does not always correspond to increased SMA expression suggests that DNA methylation may not directly act to induce BSMC de-differentiation, though it may influence both proliferation on DNC and differentiation by SHH. Future work will examine how expression of other SMC markers is affected by shRNA against specific DNMTs in a context specific manner. |
| Databáze: | OpenAIRE |
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