Comparative Efficacy of Antigen and Antibody Detection Tests for Human Trichinellosis
| Autor: | H.R. Gamble, D Ivanoska, K.D. Murrell, K Cuperlović |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Immunoradiometric assay
medicine.diagnostic_test medicine.drug_class Trichinella spiralis Radioimmunoassay Biology Trichinosis Monoclonal antibody medicine.disease biology.organism_classification Virology Antigen Immunoassay Immunology medicine biology.protein Parasitology Antibody Ecology Evolution Behavior and Systematics |
| Zdroj: | The Journal of Parasitology. 75:38 |
| ISSN: | 0022-3395 |
| DOI: | 10.2307/3282932 |
| Popis: | Sera collected from patients with suspected or confirmed exposure to Trichinella spiralis were tested for circulating parasite antigens and antiparasite antibodies. Using an immunoradiometric assay, excretory--secretory antigens from muscle-stage larvae of T. spiralis were detected in the sera of 47% of 62 patients with clinical trichinellosis and 13% of 39 patients without clinical signs but suspected of exposure to infected meat. In comparison, antibodies were detected using an indirect immunofluorescent test in the circulation of 100% of the 62 patients with clinical trichinellosis and 46% of the 39 patients with suspected exposure. The presence of antibodies specific to excretory-secretory products of T. spiralis muscle larvae was confirmed in the majority of the samples tested by a monoclonal antibody-based competitive inhibition assay. These results indicate that antibody detection is a more sensitive diagnostic method for human trichinellosis, but that antigen detection might be a useful confirmatory test because it is a direct demonstration of parasite products in the circulation. |
| Databáze: | OpenAIRE |
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