Purification and analysis of partially alkylated cyclodextrins by liquid and gas chromatography

Autor:	H. Husmann, Frank Kobor, Gerhard Schomburg, Alfred Deege, E. Hübinger
Rok vydání:	1993
Předmět:	chemistry.chemical_classification Chromatography Cyclodextrin Silylation General Chemical Engineering Fast atom bombardment Mass spectrometry High-performance liquid chromatography chemistry.chemical_compound chemistry Organic chemistry Gas chromatography Selectivity Derivatization
Zdroj:	Journal of High Resolution Chromatography. 16:587-593
ISSN:	1521-4168 0935-6304
Popis:	Complex mixtures of partially alkylated cyclodextrins can be analyzed by both HPLC and high temperature capillary GC. Because of the limited efficiency of LC, suitable analytical and preparative separations can be achieved only with systems of carefully optimized selectivity. Using LC it has been possible to isolate and purify single cyclodextrin species from very complex mixtures of components which contain unreacted hydroxyl groups in addition to the alkoxy groups. Analysis of the reaction mixtures and of fractions taken from LC separations can be performed with advantage by high resolution capillary GC at high temperatures between 300 and 400 °C. The thermal stability of partially alkylated cyclodextrins in high temperature GC is considerably increased by trimethylsilylation of the free hydroxyl groups. Fast atom bombardment mass spectrometry and proton NMR were used to identify species isolated from the preparative LC separations.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::207bfe71a48587e24b5cbb5ec5cb2d93 https://doi.org/10.1002/jhrc.1240161004 Zobrazit plný text záznamu Plný text