Engineered Liver Tissue Culture in anIn VitroTubular Perfusion System
| Autor: | John P. Fisher, Charlotte M. Piard, Jenny Katsnelson, Trevor Mollot, Daniel Rivkin, Guang Yang, Daniel Najafali, Bhushan Mahadik, Athenia Jones, Alexis Robinson, Julia Pinsky |
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| Rok vydání: | 2020 |
| Předmět: |
0303 health sciences
Chemistry 0206 medical engineering Mesenchymal stem cell Biomedical Engineering Bioengineering 02 engineering and technology medicine.disease 020601 biomedical engineering Biochemistry Cell biology Biomaterials Transplantation 03 medical and health sciences Liver disease Perfusion Culture Tissue engineering Parenchyma medicine Liver function Perfusion 030304 developmental biology |
| Zdroj: | Tissue Engineering Part A. 26:1369-1377 |
| ISSN: | 1937-335X 1937-3341 |
| DOI: | 10.1089/ten.tea.2020.0213 |
| Popis: | Liver disease and the subsequent loss of liver function is an enormous clinical challenge. A severe shortage of donor liver tissue greatly limits patients' options for a timely transplantation. Tissue engineering approaches offer a promising alternative to organ transplantation by engineering artificial implantable tissues. We have established a platform of cell-laden microbeads as basic building blocks to assemble macroscopic tissues via different mechanisms. This modular fabrication strategy possesses great potential for liver tissue engineering in a bottom-up manner. In this study, we encapsulated human hepatocytes into microbeads presenting a favorable microenvironment consisting of collagen and mesenchymal stem cells, and then we perfused the beads in a three-dimensional printed tubular perfusion bioreactor that promoted oxygen and medium diffusion to the impregnated cells. We noted high cell vitality and retention of parenchymal cell functionality for up to 30 days in this culture system. Our engineering-based approach led to the advancement in tissue size and long-term functionality of an artificial liver tissue in vitro. |
| Databáze: | OpenAIRE |
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